Expert mode
Select versus mode, highlight a CPU player, then hold L and press A (for Hard difficulty) or B (for Super hard difficulty).
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Metal Mario
Successfully complete story mode as Wario under the normal, hard, or super hard difficulty setting without losing or using the redo option. Defeat Metal Mario without losing to unlock him in versus mode.
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More viruses
Press L or R in marathon mode to increase the number of viruses.
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Super hard difficulty
Select story mode, highlight the "Hard" difficulty setting, then press Z.
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Vampire Wario
Successfully complete story mode as Dr. Mario under the normal, hard, or super hard difficulty setting without losing or using the redo option. Defeat Vampire Wario without losing to unlock him Mario in versus mode.
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